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White Papers

Company	White Paper

ATCC	Antibiotic resistance is a serious, globally extensive problem that significantly affects patient morbidity and mortality. Here, we discuss the emerging threat of antibiotic resistance and the importance of clinically relevant reference materials for use in assay development and drug discovery. Read more>>

MilliporeSigma	Recombinant eSpCas9 Protein - For RNP-Based Genome Editing The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was discovered in bacteria, where it functions as an adaptive immune system against invading viral and plasmid DNA. In this system, short DNA sequences (spacers) from invading viruses are incorporated at CRISPR loci within the bacterial genome and serve as memory of previous infections.

Battelle	Detecting Neurobehavioral Effects Neurobehavioral testing provides critical insights into how drugs and chemicals affect the nervous system. This Whitepaper covers the considerations in the Design and Execution of Neurobehavioral Studies.

ProZyme	Comparison of common fluorescent labels for liquid chromatography analysis of released N-linked Glycans Glycosylation of biotherapeutics is a frequently monitored critical quality attribute. N-Glycan analysis typically involves release and derivatization to a fluorescent label. Workflow and analysis are impacted by label choice. Click for more

INTEGRA	Pipette Routine Check – Best Practices Pipettes should be calibrated annually to maintain their perfomance. In the interim, it is recommended to perform a routine check to test the functionality of your pipettes in order to be confident with your pipetting results. Follow the guidelines below to properly perform such a routine check.

Eppendorf	The Tip of the Iceberg: How Pipette Tips Influence Results This study including tips from 15 different manufacturers proves that a pipetting system working perfectly with a certain tip exceeds the permissible error tolerances when a tip from a different manufacturer is used.

R&D Systems	A Novel Method for Detecting Terminal Glycans Using SDS-PAGE We have developed a novel technique capable of probing terminal monosaccharides on proteins using SDS-PAGE. This technique does not require expensive equipment and is more specific than comparable methods. Additionally, it offers any laboratory the ability to gain a greater understanding of the glycan profiles of their protein of interest without significant investments.

BioTek	Monitoring Viral Infection of Mammalian Cells using Digital Fluorescence Microscopy Here we demonstrate the ability of the Cytation™ 5 Cell Imaging Multi-Mode Reader and Gen5™ Microplate Reader and Imager Software to determine infection levels of HIV with HeLa cells in culture.

Hera BioLabs	Novel gene edited rat models exhibit enhanced immunodeficiency and engraftment of human tumors. Rats can produce larger samples for analysis and in many ways are more physiologically similar to humans than mice. Applications discussed include: efficacy and toxicity in a single animal, more precise surgical manipulations and better in-vivo imaging

BioTek	The imaging and analysis of fluorescently stained cells has traditionally been accomplished using manual microscopic methods with low numbers of samples. Here we describe the use of the Lionheart™ FX Automated Live Cell Imager and the CellASIC ONIX2 perfusion micro-incubator to rapidly image and analyze perfused tissue culture cells in multiple fluorescent colors and brightfield.

The Jackson Labs	NSG™* and NSG™-SGM3* mice are a proven host for engraftment of human tumors or establishment of human immunity following hematopoietic stem cell transplantation. Here, we provide evidence that NSG and NSG-SGM3 mice...(click for more)

BioStatus Limited	Real-Time Cell Health Monitoring, Cell-by-Cell, in 2D and 3D in the Far-Red. In flow cytometry and fluorescence microscopy (including high content screening), a reliable estimation of cell viability is important because it is central to assays for apoptosis and in vitro toxicology. Likewise, it is a measure ... (click for more)

Swift Biosciences	Next-generation sequencing (NGS) of bisulfite-converted DNA to detect methylation status with per-base resolution is currently restricted by input requirements, requiring at least 50 ng of DNA. This Application Note shows how the unique chemistry of the Accel-NGS® Methyl-Seq DNA Library Kit enables the construction of high complexity libraries for ....(click for more)

TTP Labtech	Multiplexed fluorescence ELISA assays on mirrorball® Enzyme-linked immunosorbant assays (ELISA) can be used in multiple stages of the drug discovery process to help identify and measure the cellular responses to therapeutic or toxic molecules.....(click for more)

BioTek	Validation of High-Throughput Wound Healing Assay using 3D Cell Patterning and Automated, Kinetic Imaging Here, we demonstrate a novel 3D wound healing assay model that can overcome 2D assay limitations. The method incorporates magnetic levitation (click for more)...

Agilent Technologies	White Paper: Why Reversed-Phased? Protein biopharmaceuticals are very heterogeneous, so a number of chromatographic techniques may be required to fully characterize and a ctive pharmaceutical ingredient (API). Methods include size exclusion chromatography for the quantification of dimers and aggregates, and ion-exchange for the indentification of charge variants. Click for more…

Agilent Technologies	Application Note: Biologics and Biosimilars Size exclusion chromatography (SEC) is an important tool for monitoring monomers, dimers, aggregates, and potential degradants in biotherapeutic protein samples, including monoclonal antibodies and their derivatives. As aggregation is considered a critical quality attribute, quantitation is required. Click for more...

Agilent Technologies	Application Note: Biologics and Biosimilars Monoclonal antibody aggregation can arise from multiple mechanisms, including product and environmental factors. Size exclusion chromatography (SEC) is a standard method to determine and quantify aggregation and fragment levels of monoclonal based therapeutics. Click for more...

Agilent Technologies	Application Note: Biotherapeutics and Biosimilars N-linked glycosylation is a critically important and very complex post-translational modification. It therefore needs to be controlled and monitored throughout development, processing, and manufacture of drug glycoproteins. Click for more...

INTEGRA	Simultaneous 96-well Extraction of Urine Samples for Drugs of Abuse Analysis INTEGRA, in conjunction with DPX Labs LLC, has produced an application note that describes a novel high throughput semi-automated sample method for processing urine samples ready for drugs of abuse analysis by LC-MS/MS. Using an INTEGRA VIAFLO 96 electronic pipette with DPX mixed mode tips, a single 96-well plate loaded with samples can be extracted and ready for LC-MS/MS analysis in less than ten minutes eliminating the need for a time-consuming evaporation step. For a copy of this new application note please click here .

Jackson Labs	THE NEXT FRONTIER IN HUMAN DISEASE MODELING JAX® offers humanized NSG™ and NSG™-based strains with additional manipulations of the host (including the NSG™-SGM3 strain that expresses the human cytokinesIL-3, GM-CSF, and SCF) for an enhanced ability to recapitulate specific functions ofhuman disease in vivo.


Trevigen	Trevigen’s Cultrex® Basement Membrane Extract (BME)for in vitro Organoid/Spheroid Studies There is a growing recognition of the role of the extracellular matrix (ECM). The ECM is the biologically active macromolecular component present in all tissues and organs. It not only provides structural support for tissues and organs but contains information that controls cellular programs, differentiation, and morphogenesis. It is not surprising that different tissues and organs contain different ECMs

EMD Millipore	Application Note: High resolution imaging flow cytometry provides comprehensive analysis of live mixed algae cultures and assesses high value commodities in algal biomass. Haley R. Pugsley and Benjamin E. Alderete

Jackson Labs	NSG™* mice are a proven host for engraftment of human tumors or establishment of human immunity following hematopoietic stem cell transplantation. Here, we provide evidence that humanized NSG mice can support the growth of allogeneic human tumors.

EMD Millipore	ImageStream® X Measurement of immune cell function using ImageStream® X Cytometry Abstract Cells of the innate and adaptive immune system defend the host from pathogens while remaining tolerant to self antigens, and dysregulation of the immune system can result in autoimmunity or infection. Immune cells sense their environment by binding and processing soluble mediators of inflammation or through direct contact with other cells, resulting in signal transduction and activation or suppression of effect or function.

Thermo Fisher Invitrogen	Many biological science applications require an accurate, precise measurement of the number of cells and their viability prior to downstream analysis. This assessment can be done manually with a traditional hemocyotmeter and microscope or with an automated cell counter, such as the Invitrogen™ Countess™ II FL.

ProteinSimple	Computer-aided Assay Development for Charge Heterogeneity Analysis by iCE The goal of this note is to promote the successful application of DOE tools in the assay development process by offering a stepwise example. The road map contained in the following pages has purposely catured enough technical detail to provide a comprehensive reference guide for both the statistician and analytical biochemist. The subjects that will be covered include initial factor screening, construction of a central composite DOE, response surface modeling, assay optimization, model validation and assay performance.

EMD Millipore	Application Note: Measuring autophagic flux by assessing LC3, p62 and LAMP1 co-localization in Jurkat cells using imaging flow cytometry. Haley R. Pugsley, Vidya Venkatachalam, Sherree L. Friend, Raymond Kong, Shobana Vaidyanathan, Phil Morrissey, EMD Millipore

Jackson Labs	Humanized NSGTM* mice are used by researchers and drug discovery scientists as powerful tools to study hematopoiesis, inflammatory disease, viral host-pathogen interactions, and are accelerating the development of novel therapies in oncology. How to select the appropriate model for your research are important considerations for conducting impactful research using humanized mice and are the focus of this paper.

INTEGRA	Handheld Pipetting - Best Practices For the vast number of scientists that undertake handheld pipetting protocols - INTEGRA has produced a highly informative illustrated 'Best Practices' guide. Drawing upon decades of pipetting experience - INTEGRA has compiled an unbiased series of hints and tips to help you improve your handheld pipetting. Expert advice is provided in areas including before you start, how to pipette and optimising pipetting performance. Each area of best pipetting practice discussed is backed up with an explanation of why this produces optimal results.

Gilson	Commonly used air displacement pipettes are highly accurate for most liquid dispensing applications. However, the viscosity, volatility, surface tension, and temperature of the solution can adversely affect the performance of these air displacement pipettes. Click here to read more

TREVIGEN	Trevigen’s Extracellular Matrix Proteins for Triculture of Breast Cancer Spheroids, Endothelial Tubules, and Human Mesenchymal Stem Cells Here we describe the 3D triculture model which recapitulates the breast cancer tumor microenvironment. Such a model will be useful for drug screening, basic research, and personalizedmedicine providing one of many needed tools to facilitate more physiologically relevant assessmentsof cancer cell behavior.

	Register for the Illumina Genomics Symposium Next-Generation Sequencing Applications for Neurological Disorders Today, cell biologists are looking to new methods, like next-generation sequencing, to investigate the genetic underpinnings of disease biology and generate hypotheses for further studies that analyze gene expression and protein function.

	New paper Breaking the Bottleneck: Boosting Lab Performance with Automated Plate Seal Removal Removing and/or replacing hundreds of plate seals at a time can represent a formidable and painstaking challenge. This paper reviews how high-throughput screening (HTS) and high-content screening (HCS) laboratories discovered hidden productivity improvements using new adhesive-based de-sealing technology.

Gilson, Inc.	Streamlined Purification of Assay-ready Oligonucleotides by Automated HPLC High Purity Oligonucleotides are used for standard molecular biology techniques such as qPCR, Next Generation Sequencing, and microarray analysis. Full-length products need to be separated from truncated sequences, inhibiting salts, and base protecting groups. Gilson has developed a liquid handling platform configured with automated preparative and analytical HPLC capabilities to isolate, analyze, and desalt reagent-grade oligonucleotides in one continuous run..

GenScript	Gene Synthesis Handbook: Applications and Technologies for DNA synthesis 36-page technical introduction to designing, constructing, and using synthetic genes. What is gene synthesis? How does it differ from traditional molecular cloning? Why is gene synthesis a revolutionary tool, and how is it performed? Includes: case studies from cancer, structural biology, vaccine development, neuroscience, plant biology, and synthetic biology; oligo design tools and assembly methods; and 54 peer-reviewed references.

TREVIGEN	Trevigen’s γ-H2AX Pharmacodynamic Assay Here we describe an ELISA based Pharmacodynamic Assay (PDA) to measure the formation of γ-H2AX. Such an assay will be useful for drug screening, basic research and upcoming clinical trials providing one of many needed tools to support hypothesis-driven drug design strategies.

	The Effect of Cell Culture Method on Long-Term Primary Hepatocyte Cell Health BioTek Instruments, Inc. Features BioTek’s MultiFlo™ FX Microplate Dispenser and BioTek’s Cytation™ 3 Cell Imaging Multi-Mode Microplate Reader Cytation™ 3 Cell Imaging Multi-Mode Microplate Reader Cytation™ 3 is a cell imaging multi-mode microplate reader that combines automated digital microscopy and conventional microplate detection. This patent pending design provides phenotypic cellular information with well-based intensity data. MultiFlo™ FX Microplate Dispenser MultiFlo FX is a modular reagent dispenser for 6- to 1536-well plates, dispensing volumes as low as 500 nL. An available wash module adds multi-purpose functionality.

Quanterix Corporation	Scientific Principle of Simoa™ (Single Molecule Array) Technology Quanterix' ultra-sensitive diagnostic platform, the Simoa HD-1 Analyzer is capable of measuring individual proteins at concentrations 1000 times lower than the best immunoassays available today. The Single Molecule Array (Simoa™) technology at the heart of the platform enables the detection and quantification of biomarkers previously difficult or impossible to measure.

Bio-Rad Laboratories	The story of Leukemia research is being re-written—one droplet at a time. See how droplet digital PCR gives scientists the power to detect what was previously undetectable. Watch Video

Eppendorf	Magnetic Bead Based Soil DNA Isolation using the epMotion® and ClearMag™ Technology Many nucleic acid purification applications have been previously automated on the Eppendorf epMotion 5075. The exception is purification of DNA from difficult samples high in PCR inhibitors, including soil, turbid water, biofilms, and human gut samples. While highthroughput purification of DNA can be achieved using 96-well silica plates, this method is time-consuming and not hands-free. This Application Note describes the successful automation of DNA isolation from these sample types with magnetic bead-based technology from MO BIO Laboratories.

BioTek Instruments, Inc.	Automation of HTRF® elF4E Kinase Assay Using a 3D Tumoroid-Based Cell Model MultiFlo™ FX is a fully modular, automated reagent dispenser for 6- to 1536-well plates offering BioTek’s unique Parallel Dispense™ technology - both peristaltic and syringe pump operation down to 500 nL. An available microplate wash module adds multi-purpose functionality. A fully configured MultiFlo FX replaces up to five liquid handlers.

Quanterix Corporation	Scientific Principle of Simoa™ (Single Molecule Array) Technology Quanterix' ultra-sensitive diagnostic platform, the Simoa HD-1 Analyzer is capable of measuring individual proteins at concentrations 1000 times lower than the best immunoassays available today. The Single Molecule Array (Simoa™) technology at the heart of the platform enables the detection and quantification of biomarkers previously difficult or impossible to measure.

ATCC	Knowledge is Power: Why Using ATCC’s Annotated, Authenticated Tumor Cell Lines for Cancer Research Matters. The availability of sequencing data is making it possible to use cell lines to tie oncogenic events to their underlying genetic cause and to examine cancer from new perspectives. However, given the potential cost of using cross-contaminated materials it is important to start your experiments with authenticated tumor cell lines or panels, such as those available from ATCC. More info at 703-365-2700 Tech@atcc.org

BioTek Instruments	Multiplexed Assay for IL-6 Secretion and Cell Viability Using an Epithelial Ovarian Cancer Cell Line Using the Cytation™3 Cell Imaging Multi-Mode Reader to Monitor IL-6 Secretion Using HTRF Detection and Cell Viability Using Digital Widefield Fluorescence Microscopy

Eppendorf	Eppendorf ThermoMixerTM C – Features, Options, Applications Katrin Käppler-Hanno, Eppendorf AG, Hamburg, Germany Executive Summary Primarily used in the field of life sciences, the Eppendorf ThermoMixerTM C is a device for the temperature control and mixing of liquids or samples. The device offers a wide temperature control range (1 °C–100 °C), high flexibility with the use of diverse tube and plate formats and advanced mixing performance with the 2DMix-Control technology and a mixing speed of 3000 rpm. With the new technology condens.protect®, the Eppendorf ThermoTop® prevents condensation on tube lids or plate sealing foils.

SDIX^®	SDIX ^® mAb Discovery Engine SDIX^® has developed an antibody engine (mAb discovery engine) for discovery of antibodies to transmembrane proteins like G-protein coupled receptors (GPCRs). The mAb discovery engine generates panels of diverse antibodies in terms of sequence, somatic hypermutation, epitopes, and performance. The mAb discovery engine is a platform that is capable of routinely generating antibody candidates that are diverse, functional and novel.

Mirus Bio, LLC	From Reprogramming to Differentiation – Transfection Applications for Stem Cell Research Differentiation and reprogramming techniques are imperative in stem cell research and require virus-free applications for biotherapeutic and new drug development. Mirus Bio presents a suite of TransIT® Transfection Reagents and an Ingenio® Electroporation Kit that are high efficiency, low toxicity DNA and RNA delivery solutions validated for a variety of stem cell applications.

Singulex	Application Note: Cardiotoxicity This application note describes the utility of the Erenna Immunoassay System and the Erenna cTnI Assay towards early assessment of cardiotoxicity in rats. Due to its unique highdefinition technology, elevated levels of cTnI in serum can be detected earlier with the Erenna Immunoassay System

Hamilton Company	Rapid, High Throughput Extraction and Purification of Genomic DNA from Whole Blood Using the Akonni TruTip® Extraction System on the Hamilton Microlab® STAR Liquid Handling System Akonni Biosystems has developed the simple and effective ultra-rapid TruTip® automated extraction method designed to isolate genomic DNA from low volumes of whole blood for downstream genetic testing including any type of PCR, sequencing and microarray detection. The tip-based extraction technology is paired with the advanced, proven and reliable Hamilton Microlab STAR liquid handling system for high throughput sample processing.

Applied Photophysics Ltd	Automated Circular Dichroism with Dynamic Multimode Spectroscopy: a powerful tool for establishing conformational stability of cloned proteinsn Abstract The relative stabilities of the 9th-10th type III domain pair of fibronectin (FIII9-10) and two mutants thereof, all of which have immunoglobulin-like beta-sandwich domains, were investigated using dynamic multimode spectroscopy (DMS) with automated circular dichroism (ACD). It is shown that the mutants are significantly more stable than the wild-type, that the folded and unfolded protein conformations for all variants are similar, and that aggregation accompanies unfolding.

Waters	Guidelines for Routine Use and Maintenance of Ultra-Performance Size-Exclusion and Ion-Exchange Chromatography Systems By: Paula Hong and Kenneth J. Fountain To decrease the frequency of system repairs and contamination, a series of steps have been outlined for maintenance and care of a chromatographic system and columns used for the analysis of biomolecules.

AMRI	Evolving Intelligence By Wendy Quinn-Decatur, M.S.I.S., Associate Librarian, AMRI As Contract Research and Contract Manufacturing Organizations (CROs and CMOs) continue to evolve and provide increasingly important contributions to their customer’s discovery and development priorities, implementing a customer-focused approach to corporate library services can turn a repository for dated literature into an invaluable information center for the library’s constituents.

Waters Corp.	Application Note Metabolite Identification Workflows: A Flexible Approach to Data Analysis and Reporting

Gilson, Inc	Analyzing catecholamines and their metabolites in patient urine samples is important for sympathoadrenal system tumor diagnosis. This application note discusses the automation of sample preparation and solid phase extraction (SPE) with the CIinRep Complete Kit (RECIPE Chemicals + Instruments GmbH) using the Gilson ASPECTMXL or GX-274 Liquid Handler. Following SPE, fractions were analyzed via HPLC for analytes VMA, HVA, and 5-HIAA using an electrochemical detection (ECD) method.

Applied Photophysics Ltd	CHIRASCAN SERIES APPLICATION NOTE: Protein Stability by Chemical Denaturation Abstract Automated Circular Dichroism (ACD) was used to determine the thermodynamic stability of a protein by chemical denaturation. ► Incubation of samples ensures equilibrium is reached, eliminating systematic error ► Short path lengths extend the accessible wavelength range ► Automation significantly increases productivity and reduces human error

INDIGO Biosciences, Inc.	Retinoid Receptors: Overview and Tools for Researchers 　 Retinoids are a class of chemical compounds related to vitamin A. The two predominant and most studied retinoids are all-trans retinoic acid (ATRA) (the acid form of vitamin A also known as Tretinoin) and 9-cis-retinoic acid (9-cis RA or Alitretinoin). Retinoids have many important and diverse functions throughout the body, including roles....

Bio-Rad Laboratories	Effect of Conductivity on DBC on CEX Resin In an effort to improve the antibody manufacturing platform, newer cation exchange resins were evaluated in this study. Dynamic binding studies were conducted using 2 different monoclonal antibodies at a stable pH and varying conductivities across nine resins to determine the differences in binding capacity.

Shimadzu Scientific Instruments	The Perfinity Workstation: Automating Mass Spec Sample Preparation This paper presents the Perfinity Workstation, a fully-integrated online protein sample preparation workstation, combining the selectivity of immunoassay approaches with the resolution of HPLC. It utilizes multiple proteomic approaches to rapidly and reproducibly perform affinity selection, buffer exchange, trypsin digestion, desalting of the sample and reverse phase LC analysis. All of these processes enable the generation of purified peptides ready for LC-MS analysis in about 10 minutes, and result in peptide maps of exceptional reproducibility (<10% CVs).

Gilson, Inc	This application note provides a quick comparison of three pharmaceutical compounds using high pressure normal phase and low pressure flash chromatography on the Gilson PLC 2020 Personal Purification System. Comparison of peak shape, peak retention time, chromatography run time, fraction volume, and solvent consumption were all studied to determine the most efficient and optimal chromatography method.

Bio-Rad Laboratories	Composite Profiling of Angiogenic Factors Using Bio-Plex Pro Human Cancer Biomarker Panel 1 The Bio-Plex Pro human cancer biomarker panel 1 is a unique blend of magnetic bead-based multiplex assays designed to meet the needs of the most discerning preclinical and clinical researchers. The multiplex format enables robust and reproducible measurement of 16 soluble proteins involved in disease processes such as: Angiogenesis, Metastasis, Cell proliferation, Cell adhesion/migration, Apoptosis and Inflammation

Applied Photophysics Ltd	Strategies to compare far-UV Circular Dichroism spectra of similar proteins using Chirascan™-plus ACD Circular Dichroism (CD) spectroscopy is an extremely sensitive technique to analyse the conformation of proteins in solution and can be used as a fingerprint of a particular protein structure [Greenfield, 2006]. Due to the very low sample volumes required and the simplicity of the measurement, it is a powerful comparative tool to detect differences in structure between proteins. This paper discusses how Chirascan™-plus ACD presents easy-to-obtain, highly reproducible results while achieving high throughput, previously not available for Circular Dichroism. Using advanced robotics combined with the 96 or 384-well plates, Chirascan™-plus ACD can rapidly prepare many replicates and perform multiple measurements for clear determination of standard deviations.

BIOSEARCH TECHNOLOGIES	Stellaris RNA FISH: Detection, Localization, and Quantification of mRNA at the Cellular Level Presented here is a new method for in situ RNA detection, the Stellaris™ FISH technology, which enables simultaneous detection, localization, and quantification of individual RNA molecules at the cellular level. This technique provides a high degree of specificity and sensitivity, is ideal for applications that analyze transcription site activity, protein- RNA interactions and mRNA translocation events, and holds promise for future diagnostic applications.

InhibOx	Large Custom Databases Unlocking the Potential of your Chemistry Investment Too often, virtual screening has failed to deliver useful results. The data from your past projects is high quality, but may be focused on the wrong area and lack diversity. This new white paper from InhibOx describes how companies can build and rapidly search very large, high quality virtual libraries, starting from their own reagent collections and chemistries, providing a platform for successful virtual screening.

JMP Clinical	A Primer on JMP® Clinical Incidence Screens JMP Clinical has a number of analytical processes (APs) that calculate and compare the incidence of events between two or more treatment groups in a clinical trial. Interventions Incidence Screen enables you to compare the incidence of concomitant medications or substances. AE Incidence Screen enables you to compare the incidence of adverse events. DS/MH Incidence Screen enables you to compare disposition events (e.g., randomization or completion status) or medical history terms (which, technically, is a comparison of prevalence). Finally, AE Resolution Screen enables you to examine the resolution of adverse events. Most of these APs operate in a similar manner. In this white paper, we use AE Incidence Screen with sample data to illustrate the common functionality of these APs. We also point out important differences among the other APs at the end of the example

Lucigen Corporation	Expresso^® Cloning and Protein Expression Systems enable you to effortlessly clone your gene and express protein the next day. PCR amplicons are directly mixed with cloning-ready vectors and competent cells to yield >90% recombinants, with no ligation step. Expression is tightly controlled. The optional SUMO fusion tag increases solubility.

EMD Millipore	The Importance of Being Profiled: Improving Drug Candidate Safety and Efficacy Using Cross-target Profiling Profiling of putative lead compounds against a representative panel of important enzymes, receptors, ion channels and transporters is an excellent way to establish a preliminary view of potential issues that might later hamper development. An early idea of which off-target activities must be minimized can save valuable time and money during the preclinical lead optimization phase if pivotal questions are asked beyond the usual profiling at hERG. The best data for critical evaluation of activity at GPCRs and ion channels is obtained using functional assays, since binding assays cannot detect all interactions and do not provide information on whether the interaction is that of an agonist, antagonist, or allosteric modulator. This overview discusses the benefits of in vitro assays, specific decision points where profiling can be of immediate benefit, and highlights relevant profiling services offered by EMD Millipore.

DiscoveRX	DAWN OF A NEW ERA FOR KINASE DRUG DISCOVERY: KINOME WIDE PROFILING AND SCREENING SOLUTIONS COUPLED TO FUNCTIONAL CELL-BASED ASSAYS ENABLE LAUNCH OF SELECTIVE AND FUNCTIONAL KINASE INHIBITORS

INDIGO Biosciences	Live Cell Multiplex Assay For use in combination with INDIGO’s 3x32-, 2x48-, or 1x96-well format Nuclear Receptor Reporter Assay Systems

SABiosciences, a QIAGEN company	RT2 Profiler™ PCR Arrays Pathway-Focused Gene Expression Profiling with qRT-PCR This paper evaluates the performance of the newest technique for monitoring the expression of a panel of pathway- or disease-specific genes: the RT2 Profiler PCR Array System from SABiosciences. The PCR Array System combines the quantitative performance of SYBRR Green-based real-time PCR with the multiple gene profiling capabilities of a microarray. The PCR Array is a 96- or 384-well plate containing RT2 qPCR Primer Assays for a set of 84 related genes, plus five housekeeping genes, and three controls.

Trevigen, Inc	DNA Repair Deficient Cell Lines- Tools to Study Genomic Instability- White Paper DNA repair proteins maintain the stability of the genome. When repair protein function is impaired through mutations, the genome may become unstable which is a hall mark of solid tumors. Trevigen, Inc. is pleased to announce the availability of nineteen DNA repair gene knockdown cell lines, encompassing the Base Excision Repair pathway. The availability of this panel of target specific LN428 glioblastoma shRNA lentivirus transduced cell lines will permit scientists to study the molecular etiology of tumor genomic instability and to exploit it in oncology research.

DiscoveRX	Propelling GPCR drug Discovery through innovative products Focus: Cutting edge portfolio and how it has led to new learnings

Caliper Life Sciences	Fragment-Based Screening using the LabChip EZ Reader - White Paper Introduction Fragment-based lead discovery is establishing itself as valuable approach for the discovery of novel small molecule leads. It relies on establishing a better understanding of the binding mechanism of a ligand for its binding partner by probing for “hot spots “on the protein target with small molecule fragments (typically <300 Daltons). Its simplicity is in the principle of building a pharmacophore from the ground up. Successful selection requires data on binding affinity as well as extensive SAR information provided by a variety of techniques . . .

Caliper Discovery Alliances & Services	CDAS has created GPCRFunctional™ Label Free service programs to offer more relevant assay development, screening, drug activity and receptor characterization studies in a more physiological cellular context.

Ariadne Genomics	Mechanistic Disease Modeling Developing mechanistic models that can help drive hypotheses and explain disease and its progression is a challenge for drug discovery and development companies. This white paper discusses how an in silico approach to defining punitive disease models can assist researchers in their efforts to find novel drug targets, elucidate drug toxicities and find new indications for existing therapies.

Gilson, Inc	Pipetting Hot and Cold

SABiosciences, a QIAGEN company	PCR Array: A Simple and Quantitative Method for Gene Expression Profiling The RT² Profiler™ PCR Array System is the most reliable and sensitive gene expression profiling technology for analyzing a panel of genes in signal transduction pathways, biological processes, or disease related gene networks. From Sample Preparation to Data Analysis, the PCR Array system employs four components that Guarantee assay performance, yielding high-quality, reproducible, and reliable gene expression data.

Accuri Cytometers, Inc.	The Accuri^® C6 Flow Cytometer^® represents a dramatic step forward in flow cytometry and cell analysis. Now researchers have access to a high-capability analytical cytometer at an affordable price. The benchtop-sized C6 makes a powerful research tool available to a much wider range of life scientists. This article examines a variety of biological applications that take advantage of the C6’s cellular analysis capabilities.

MD Biosciences, Inc	Progressing pipelines withPre-clinical CRO Partnerships. A case study in successful outsourcing to meet objectives in licensing/acquisitions, repositioning/therapeutic expansion and customized applications for screening of NCE/biologics.

Invitrogen by Life Technologies	Measure inhibitor binding to non-activated kinases The LanthaScreen® Eu Kinase Binding Assay is an easy to optimize kinase assay for screening and multiple downstream application. This modular assay format has been validated for use with over 200 kinase targets. In this application note, we present examples of compound affinity comparisons for active and non-active kinase forms, including kinase inhibitors with various binding mechanisms

Accuri Cytometers, Inc.	A Powerful and Cost Effective Solution for Today’s Research Laboratory The Accuri C6 Flow Cytometer^®System represents a dramatic step forward in flow cytometry.

BioTek Instruments, Inc.	Automated Magnetic Bead Washing of Metabolic Hormone Assays Using the ELx405™ Magnetic Bead Washer to Wash MILLIPLEX™ MAP Assays

Invitrogen by Life Technologies	Simple off-rate measurements with the LanthaScreen® Eu Kinase Binding Assay The LanthaScreen® Eu Kinase Binding Assay is an easy to optimize kinase assay for screening and multiple downstream application. This modular assay format has been validated for use with over 200 kinase targets. In this application note, we present a simple for measuring inhibitor off-rates.

SABiosciences	Biomarker Discovery from Paraffin Embedded Samples- Successful Principles for Addressing FFPE-associated Real-time PCR Challenges

R&D Systems, Inc.	Using Proteome Profi ler™ Antibody Macroarrays to Assess Kinase Phosphorylation and Activation

Millipore	Unlocking the Mystery of Cancer: Do the Mutant p110α Subunits of PI3-Kinase Hold the Key?

R&D Systems, Inc.	Using Cell-Based ELISAs to Measure the Levels of Two Proteins Simultaneously in Whole Cells

Trevigen, Inc.	2^nd Generation PARP in vivo Pharmacodynamic Assay: In this article we present information regarding Trevigen’s 2nd Generation PARP Pharmacodynamic Assay. We provide validation data and accelerated shelf life data regarding key assay components for the new assay format which features: a 96 well assay plate pre-coated with PAR capture antibody, extended dynamic range, and high signal to noise ratios.

SANYO Biomedical	Use of Narrow Bandwidth Ultraviolet Light and Copper-Enriched Stainless Steel Construction to Achieve Active Background Contamination Control in a Cell Culture CO₂ Incubator (1.9 MB pdf file)

Caliper Life Sciences	Faster Innovation Cycles from Microfluidics Technology (103kb pdf file)

The Baker Company	Terminology Changes in the 2002 Revision of NSF 49 NSF/ANSI Standard #49 - 2002 Class II Biological Safety Cabinets Types (62kb pdf file)

Back West, Inc. and Jumpstart Biodevelopment	Significant advantage can be gained by organizations utilizing high performing virtual teams working with early stage drug development projects. This paper chronicles the experiences of Dr. Whitehouse and Mr. Smith with such high performing virtual teams in early stage drug development projects and provides lessons to others seeking to deploy similar models.

AstraZeneca LP	Emerging Business and Operational Models In The Pharmaceutical Industry (69KB pdf file)

Adeptia Inc.	Business Process Management (BPM) has emerged as a proven technology that helps companies meet objectives and gain competitive advantage.