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Pierce SILAC protein quantitation kits
June 2007
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Thermo Fisher Scientific Inc. introduces Thermo Scientific Pierce SILAC (stable isotope labeling with amino acids in cell culture) protein quantitation kits for comprehensive identification, characterization and quantitation of proteins. The new kits allow for isotopic labeling of the proteome for the analysis of relative protein abundance by mass spectrometry, providing an all-in-one solution. The Pierce SILAC protein quantitation kits provide in vivo labeling that incorporates 13C- or 15N-labeled amino acids directly into proteins using normal cellular metabolism of living cells. This strategy provides 100 percent label incorporation into proteins prior to digestion. These kits include Thermo Scientific HyClone media, heavy and light amino acids, and HyClone dialyzed serum in a single convenient package. The Pierce SILAC Kits enable quantitation of protein expression levels between differentially treated cell populations. This is made possible because the heavy and light amino acids are chemically identical to each other, yet isotopically distinct.  By employing high-resolution analysis with the Thermo Scientific LTQ-FT Ultra and LTQ Orbitrap, the differentially expressed proteins are detected by high resolution MS analysis based on relative peak intensity
 
Thermo Fisher Scientific Inc.
800-874-3723

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