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Highly Flexible microRNA Library Creation
August 2007
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Integrated DNA Technologies (IDT) has announced the availability of its new proprietary miRCat small RNA cloning system. The kit-based resources make the creation of small RNA (including miRNAs, piRNAs and endogenous si-RNAs) libraries from any primary RNA source, highly time- and cost-efficient with peerless flexibility and consistency. The miRCat system permits cloning of very rare small RNAs and takes into account the natural variability in structure and sequence between species. It is compatible with most existing standard laboratory protocols for processes such as RNA extraction, purification and cloning. The miRCat cloning system consists of three sequential protocols: RNA isolation and enrichment, followed by cloning linker attachment, and ending with amplification and cloning. The system can be used for concatamer cloning or direct 'shotgun' cloning. For cloning small RNAs lacking the 5'-phosphorylated end present in miRNAs, a modified kit—miRCat-33—has been developed, which uses 5' ligation-independent cloning.
 
Integrated DNA Technologies
800-328-2661

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