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Flexible, Dynamic Live-Cell Research
November 2007
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The Leica AM TIRF MC (multi-color, total internal reflection fluorescence) system allows the entire fluorescence excitation spectrum to be used for real-time visualization of live-cell dynamics. Leica's scanner sensor automatically matches the TIRF angle at all wavelengths to the required penetration depth, and positions the laser so that the TIRF penetration depth remains constant even when the wavelength is changed. The Leica AM TIRF MC integrates four solid-state lasers for the excitation of fluorophores at wavelengths from 405 to 632nm. The system features extremely short switching times and an ultra-high synchronized frame rate. The Leica AM TIRF MC provides wavelengths of 405, 488, 561, and 632nm for multi-color applications. Individual laser lines are paired and controlled quickly and precisely via AOTF (acousto-optical tunable filters) during a switching time of only 1 ms. Leica's newly designed SyncBoard controls the fast frame rate of up to 30 fps. This high temporal resolution for the excitation of multiple fluorophores, combined with the high spatial TIRF resolution of 70-300nm in the z-axis, are key prerequisites for visualizing transport processes in cell membranes or protein interactions in real time.
Leica Microsystems Inc.
800-248-0123

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