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One-step Cell Lysis and Protein Purification, Sigma-Aldrich, Corp.
February 2006
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Sigma, a division of Sigma-Aldrich Corporation has launched a new HIS-Select™ iLAP™ 5 ml Column (Product code H9913). The HIS-Select iLAP column is an innovative method that combines cell lysis and histidine- tagged protein purification steps into one step, allowing fast, efficient and non-denaturing protein extraction directly from microbial cultures. The HIS-Select iLAP column consists of a novel single-use, disposable column designed for the one-step purification of histidine-tagged proteins directly from a 5 ml bacterial culture. This product maximizes the speed and efficiency of recombinant protein extraction while eliminating sample loss. The column contains highly specific HIS-Select™ nickel chelate matrix and highly efficient CelLytic™ Express, both present in a convenient tablet format. The column is designed for the simultaneous, direct lysis of a 5 ml bacterial culture and affinity capture of the target histidine-tagged protein without any additional manipulation required (such as centrifugation). As the culture is lysed in the column, the target protein is captured on the same highly selective HIS-Select Affinity Gel. The relatively high capacity feature of these columns enables capture of at least 1-2 mg of histidine-tagged protein per column, making them ideal for confirming expression of a histidine-tagged target protein, for performing protein:protein interaction assays, or for rapidly screening colonies or multiple constructs (cultures). The resulting purified protein is compatible with protein assays, SDS-PAGE, Western blotting and MALDI.
 
Sigma-Aldrich
800.325.5832

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