The CellPlayer NeuroPrime Cell Kit is the first commercially available cryopreserved neuronal/astrocyte co-culture kit. It is designed for long-term, kinetic live-cell measurement of neurite dynamics. Each cell kit contains cryopreserved cell vials of rat forebrain neurons and rat astrocytes and a vial of the novel, neuron-specific labeling reagent, NeuroLight Red. A fully validated protocol for thawing, labeling and measuring neurite outgrowth in a 96-well format using IncuCyte ZOOM live-cell imaging system is also included.