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Polyubiquitin protein conjugates, Pierce
September 2005
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The Pierce Ubiquitin Enrichment Kit (Product # 89899) facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples. The enriched fraction can be subsequently analyzed to determine the amount of general ubiquitin conjugates present or to identify a specific protein of interest by Western blot, or it can be used in other downstream assays and purifications. The assay protocol is fast, allowing isolation of polyubiquitinated proteins and the fractionation of mono-ubiquitinated species in the resin flow-through in less than 45 minutes. The kit contains everything needed for enriching up to 15 lysate samples containing ~0.15 mg total protein per sample.
 
Pierce
800-874-3723

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